Description
For SampleFor the quantitative determination of mouse gonadotropin-releasing hormone(GnRH) concentrations in serum, plasma, tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for GnRH and Horseradish Peroxidase (HRP) conjugated GnRH. The competitive inhibition reaction is launched between with HRP labeled GnRH and unlabeled GnRH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GnRH in the sample. The color development is stopped and the intensity of the color is measured